Omics · study · 2026
冷冻化处理和通道依赖性恢复下HeLa细胞的RNA序列分析
Listed in NCBI GEO
This study performed RNA sequencing to investigate transcriptional changes in HeLa cells under cryosilicification treatment (Si20 and Si30) and in untreated controls.
Description
Cells were profiled at early response stage (P0, 0 h and 6 h) and during passage-dependent recovery (P1–P3). Differential gene expression and pathway analyses were conducted to characterize cellular responses to cryosilicification and to evaluate the persistence of transcriptional changes across cell passages.
All samples were sequenced using Illumina NovaSeq platforms (NovaSeq 6000 and NovaSeq X Plus), and both raw sequencing data and processed expression matrices are provided.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE336nnn/GSE336961 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE336961 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1483634 ncbi.nlm.nih.gov/bioproject/PRJNA1483634 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Homo sapiens
- From keywords
- Life Sciences
- Inferred from text
- RNA sequencing 75% · Sequencing 75%
Provenance · 1 source records, 9 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE336961 | 11 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:9606 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |