Long-term efficacy of carbon dioxide laser in patients with Hailey-Hailey disease. Assessment of gene expression change. A clinical trial

We utilized the Ablative AcuPulse CO2 laser (10,600 nm) with the following parameters: Scanner F200, shape size 8 mm, depth 4, time 0.50 seconds, power 24 watts. Biopsies of healthy and affected skin were performed before treatment and 6 months post-treatment to evaluate changes in gene expression.

RNA extraction followed by molecular analysis using microarray technology and Sanger method was conducted. Additionally, blood samples were collected for mutation analysis in peripheral blood. The validated application ImitioMeasure was used for measuring the reduction of the affected area.