Integrating genome-wide association and Fst-based selection signature analyses pinpoints candidate loci for body weight in Beagle dogs

Whole-genome resequencing was performed on the DNBSEQ-T7 platform at approximately 5× depth, yielding 162,133 SNPs after quality control. The dataset includes PLINK binary genotype files (.bed, .bim, .fam), body weight phenotypes, covariates (principal components and sex), GWAS summary statistics, significant loci identified by GWAS and Fst overlap analysis, and Fst selection signature results between high- and low-weight groups.

GWAS identified ten significant loci (FDR q < 0.05) on chromosomes 6, 10, 17, 23, and 26. Overlap with Fst signals revealed six loci under selection: three intronic variants in HMGA2 on chromosome 10 and three intergenic variants between MED13L and SPRING1 on chromosome 26, reported for the first time in Beagle dogs as associated with body size. The reference genome was Dog10K_Boxer_Tasha (GCF_000002285.5) and annotation was performed with SnpEff v5.4c.