Data Sheet 1_Vaginal Nugent Lactobacillus morphotype score and endometrial pathogen detection: a two-centre retrospective study.docx

Introduction<p>This two-centre retrospective observational study investigated whether the vaginal Nugent Lactobacillus morphotype score (LMS) is associated with endometrial pathogen detection and intrauterine Lactobacillus abundance in women with infertility.</p>Methods<p>The study included 282 women (Centre A, n = 92; Centre B, n = 190) who underwent simultaneous vaginal Nugent scoring and endometrial quantitative PCR between March and December 2025.

Women who had used antibiotics within one month or any probiotics before testing were excluded. Associations were assessed by Spearman rank correlation and by multivariable logistic regression adjusted for centre, age, BMI, and recurrent implantation failure.</p>Results<p>LMS was positively associated with endometrial pathogen detection (ρ = +0.229, p < 0.001). After adjustment for centre, age, BMI, and recurrent implantation failure, each one-point increase in LMS was associated with higher odds of pathogen detection (adjusted OR 1.40, 95% CI 1.17–1.68; p < 0.001).