Omics · study · 2026
Novel Stem Cell Model Uncovers Macrophage-Mediated Antiviral Immunity and Repair in Human Type 2 Alveolar Epithelial Cells
Listed in NCBI GEO
The lung alveoli are constantly exposed to inhaled pathogens and inorganic hazards, relying on robust defence mechanisms to maintain homeostasis.
Description
Alveolar macrophages and type 2 alveolar epithelial cells (AT2s) collaborate to orchestrate protection. Compromised defence can dysregulate immunity and repair, leading to acute and chronic respiratory diseases.
To better understand these processes and drive therapeutic discovery, human model systems that capture key cell interactions are essential. Here, we develop the first induced pluripotent stem cell (iPSC)-derived platform that integrates AT2 cells and macrophages in an air-liquid interface. Coculture enhanced AT2-specific gene expression and lipid synthesis, while macrophages actively metabolised surfactant. iPSC-derived AT2s supported macrophage survival by producing M-CSF, promoting an alveolar macrophage-like phenotype.
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Additionally, macrophages played a crucial role in modulating proinflammatory signalling, enhancing antiviral immunity, and restricting viral replication during respiratory infections. Furthermore, we identify a role for iPSC-derived macrophages in epithelial repair, a process disrupted by VEGF signalling. We present an iPSC-derived platform to study AT2-macrophage interactions in homeostasis, infection, and repair, offering valuable insights into their roles in respiratory disease initiation and progression.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE294nnn/GSE294640 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE294640 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1251003 ncbi.nlm.nih.gov/bioproject/PRJNA1251003 ↗
project · from NCBI GEO
- PubMed 41842940 pubmed.ncbi.nlm.nih.gov/41842940 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Homo sapiens
- From keywords
- Life Sciences
- Inferred from text
- Disease 75%
Provenance · 1 source records, 8 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE294640 | 14 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[disease].local:disease:disease | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[organism].NCBITaxon:9606 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |