RNA-seq analysis of B16F10 cells following p300/CBO knockout

In many cancers, including melanoma, these proteins redundantly regulate key oncogenic and lineage-specific gene programs. To investigate their combined role in melanoma, we generated p300/CBP sinlge and double-knockout (dKO) B16F10 murine melanoma cells using CRISPR/Cas9.

We then performed bulk RNA-sequencing to identify genes and biological pathways that are critically dependent on these coactivators. Our results define the shared and unique transcriptional targets of p300/CBP in the B16F10 model and provide a resource for understanding how their loss impacts melanoma cell fitness, invasive potential, and stress responses.