Omics · study · 2026
The role of TPM1 isoform1.1 and 1.6 on global splicing events of mice heart
Listed in NCBI GEO
This study aimed to investigate the role of alternative splicing of TPM1 exon 9a in mice heart.
Description
We utilized adeno-associated virus serotype 9 (AAV9) vectors under the control of the cardiac troponin T (cTnT) promoter to deliver two major cardiac TPM1 isoforms—TPM1a (variant 1.1, containing 9a) and TPM1b (variant 1.6, lacking exon 9a)—into cardiomyocytes of mouse. Each mouse received 3×10¹¹ viral genomes of the respective AAV9 construct.
At the experimental endpoint, bulk RNA sequencing was performed on cardiac tissue to assess global splicing changes. Our analysis revealed that exogenous expression of either TPM1 isoforms did not significantly alter global splicing patterns.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE313nnn/GSE313175 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE313175 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1378746 ncbi.nlm.nih.gov/bioproject/PRJNA1378746 ↗
project · from NCBI GEO
- PubMed 42261667 pubmed.ncbi.nlm.nih.gov/42261667 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus · Other
- From keywords
- Life Sciences
- Inferred from text
- Heart 75% · RNA sequencing 75% · Sequencing 75%
Provenance · 1 source records, 11 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE313175 | 13 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[anatomy].local:anatomy:heart | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[method].geo_series_type:other | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |