Supplementary file 1_Longitudinal plasma proteome profiling after allogeneic hematopoietic stem cell transplantation: heterogeneous development and associations with acute GvHD.docx

It remains difficult to predict whether and when patients will develop acute GvHD. Here, we used mass-spectrometry (MS)-based proteomics to identify plasma proteins associated with allogenic HSCT and acute GvHD development.

In this study, we analyzed longitudinal plasma samples at baseline (pre-HSCT), 1, 3, 6, and 12 months post-HSCT from 10 allogeneic HSCT recipients, 7 of whom developed acute GvHD, and 5 healthy donors. As expected, significant differences between the plasma proteomes of allogeneic HSCT recipients and healthy donors were found. The most pronounced differences were observed 3 months post-HSCT, with four proteins (C4A, FTL, GNPTG, S100A9) that were altered before allogeneic HSCT and remained altered up to 12 months post-HSCT compared to healthy donors.