Omics · study · 2026
Identification RBPMS2 as a key regulator in mouse early oocyte development [RIP-seq]
Listed in NCBI GEO
In the process of follicle formation, oocyte stably maintain large number of RNAs for development.
Description
However, how mammalian oocytes regulate these mRNAs, especially in the early oocyte, is unclear. We focused on RNA-binding proteins and identified RBPMS2 as a key factor in this process.
Genetic disruption Rbpms2 causes female mouse infertility due to primordial oocyte defect. Transcriptome and RIP-seq analysis revealed that RBPMS2 binds 3′ UTR region to stabilize mRNAs of known oogenesis regulators, such as Lhx8. RBPMS2 also targets transcripts regulating cytoskeleton and cellular trafficking, such as Clasp1 and Rab27a, which are down-regulated in knock out oocytes.
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Rbpms2 knock out induces impairments in endocytosis and the accumulation of mitochondria and Golgi bodies in primordial oocyte. In summary, our study reveals RBPMS2 regulates mouse oogenesis through stabilizing mRNAs regulating oocyte development cellular trafficking and organelle enrichment, connecting post-transcriptional regulation to organelles accumulation and early oocyte development.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE316nnn/GSE316947 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE316947 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1405577 ncbi.nlm.nih.gov/bioproject/PRJNA1405577 ↗
project · from NCBI GEO
Topics
- Stated by source
- Genome binding/occupancy profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
Provenance · 1 source records, 7 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE316947 | 15 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:genome-binding-occupancy-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |