Investigating the mechanism of ferulic acid and cisplatin-containing serum inhibiting invasion of triple-negative breast cancer based on CBX2 knockdown and EGFR/PI3K pathway_raw data

Different groups of FCCS were prepared, and both CBX2 knockdown and normal MDA-MB-453 and 4T1 cells were divided into the following groups: control (Con), Con＋CBX2 of short hairpin RNA (Con+shCBX2), FCCS, FCCS+shCBX2, FCCS+shCBX2+LY294002(PI3K inhibitor), and CDDP.

After 48 hours of intervention, cell proliferation inhibition was assessed using the CCK-8 assay; apoptosis was detected by flow cytometry; cell migration and invasion were evaluated using transwell chambers; ELISA was used to measure the expression of E-cadherin, N-cadherin, and VE-cadherin in cell culture supernatants; Western blot was performed to analyze the expression of phosphorylated EGFR (p-EGFR), phosphorylated PI3K (p-PI3K), E-cadherin, and N-cadherin in cells.