Omics · study · 2026
A Cost-Effective Nuclei Isolation Protocol for Single Nucleus RNA-Sequencing of Mouse HFpEF Hearts
Listed in NCBI GEO
Single cell or single nuclei RNA-sequencing (snRNAseq) enables high-resolution profiling of cellular states, but sample preparation is often costly and complicated by debris formation and cell clumping.
Description
We describe a cost-effective approach using commercially available buffers and standard laboratory equipment to isolate high-quality nuclei from diseased cardiac tissue for reproducible downstream snRNAseq analysis. In addition, we include a previously unpublished heart failure with preserved ejection fraction (HFpEF) vs Chow dataset generated using this protocol.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE331nnn/GSE331341 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE331341 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1467710 ncbi.nlm.nih.gov/bioproject/PRJNA1467710 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
- Inferred from text
- Heart 75%
Provenance · 1 source records, 8 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE331341 | 7 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[anatomy].local:anatomy:heart | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |