Table · study · 2026
Closed form of ezrin binds RNA to maintain a metastatic phenotype in osteosarcoma
Listed in NCBI GEO
Ezrin is a cytoplasmic protein that can exist in multiple conformational forms that are regulated by phosphorylation at the threonine 567 residue.
Description
Phosphorylated open form of ezrin was generally accepted as the active form because it can translocate to the plasma membrane whereas unphosphorylated closed ezrin, sequestered in the cytoplasm, have been considered inactive. Here, we identified closed ezrin as a novel RNA binding protein with biological activity.
Our previous work established that ezrin directly interacts with cytoplasmic RNA binding proteins (RBPs). Here we show that ezrin expression correlates with RBPs in human osteosarcoma samples. Recombinant ezrin in its closed conformation directly bound RNA with 6.1 nM affinity.
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Ezrin binding specificity was highest for the guanine-rich sequences and RNA G-quadruplexes (G4 RNA). Reintroduction of closed ezrin expression rescued the transcriptome and proteome profiles in an ezrin-null osteosarcoma cell line. Closed ezrin also bound endogenous mRNAs that were enriched for pathways related to RNA processing and splicing, DNA maintenance, and cellular metabolism.
Closed ezrin also rescued the metastatic capability of osteosarcoma xenografts in zebrafish. Taken together, our data established that the closed conformation of ezrin, previously thought to be inactive, can directly bind RNA and contribute to the metastatic phenotype.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE309nnn/GSE309914 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE309914 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1363991 ncbi.nlm.nih.gov/bioproject/PRJNA1363991 ↗
project · from NCBI GEO
- PubMed 41843652 pubmed.ncbi.nlm.nih.gov/41843652 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Mus musculus · Other
- From keywords
- Life Sciences
Provenance · 1 source records, 7 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE309914 | 13 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:other | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |