Table · dataset · 2026
Investigations into factors that drive the emergence of novel influenza reassortants in pigs under field conditions
Listed in figshare and Loughborough Research Repository — shown once because both records carry DOI 10.13020/6vm1-7r16
<p dir="ltr">In order to gain insight into the level of genetic diversity and reassortment occurring in pigs, we sampled pigs throughout their production cycle from commercial farms known to be infected with multiple subtypes of influenza virus.
Description
Multiple influenza virions were isolated from single pigs by plaque purification, their genome amplified using MBTuni primers, cDNA libraries prepared using Nextera DNA XT sample preparation kit, their whole genome sequenced by Ilumina NextSeq platform and analyzed to identify whether pigs harbored one or more distinct types of influenza viruses (i.e genotypes).
A blood sample was also collected from the pigs to evaluate the pigs’ immune status against the influenza viruses present in the farm. Sera was tested by the inhibition of hemaglutination test. The protocols for animal sampling and testing were approved by the University of Minnesota IACUC and IBC.
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Information on pigs’ age, production stage and cohort was obtained. In total we investigated three cohorts of pigs, from weaning to near market, that were mixed at weaning and originated from three breeding herds. We sampled 720 pigs from which 62 influenza infected pigs yielded 428 viral plaques which after further characterization yielded 173 complete genomes and 237 incomplete genomes.
The serological analysis using the hemagglutination inhibition test to evaluate levels of protective immunity against the distinct influenza viruses known to be present in the herds were conducted against four distinct types of influenza viruses including representatives of the H1 and H3 subtypes.</p><p><br></p>
Links
Where it is published
- DOI doi.org/10.13020/6vm1-7r16 ↗
DOI / persistent id · from figshare com
Catalogue records · 1
- OAI-PMH record api.figshare.com/v2/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Af… ↗
metadata API · from figshare com
Topics
- From keywords
- Animal production · Animal production · Astronomy & Astrophysics · Biological adaptation · Biological adaptation · Chemistry · Chemistry · Computer Science & AI · Computer Science & AI · Disease surveillance · Disease surveillance · Earth & Environmental Science · Earth & Environmental Science · Economics & Finance · Economics & Finance · Engineering · Engineering · Epidemiology · Epidemiology · Evolutionary biology · Evolutionary biology · Genomics · Genomics · Humanities · Humanities · Life Sciences · Life Sciences · Materials Science · Mathematics & Statistics · Medicine & Health · Medicine & Health · Ocean & Atmospheric Science · Psychology & Behavioral Science · Public health · Public health · Social Science · Social Science · Veterinary sciences · Veterinary sciences
Provenance · 2 source records, 44 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| figshare | oai:figshare.com:article/32816456 | 7 d ago | JSON v1 |
| Loughborough Research Repository | oai:figshare.com:article/32816456 | 7 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · figshare com | connector:figshare_com@1.0.0 | |
| concepts[field].anzsrc:field:310403 | mapping · repository lboro ac uk | vocabulary-mapper@1.0.0 | keywords['Biological adaptation'] |
| concepts[field].anzsrc:field:310403 | mapping · figshare com | vocabulary-mapper@1.0.0 | keywords['Biological adaptation'] |
| concepts[field].anzsrc:field:310509 | mapping · figshare com | vocabulary-mapper@1.0.0 | keywords['Genomics'] |
| concepts[field].anzsrc:field:310509 | mapping · repository lboro ac uk | vocabulary-mapper@1.0.0 | keywords['Genomics'] |
| concepts[field].anzsrc:field:420202 | mapping · figshare com | vocabulary-mapper@1.0.0 | keywords['Disease surveillance'] |
| concepts[field].anzsrc:field:420202 | mapping · repository lboro ac uk | vocabulary-mapper@1.0.0 | keywords['Disease surveillance'] |
| concepts[field].anzsrc:group:3003 | mapping · repository lboro ac uk | vocabulary-mapper@1.0.0 | keywords['Animal production'] |
| concepts[field].anzsrc:group:3003 | mapping · figshare com | vocabulary-mapper@1.0.0 | keywords['Animal production'] |
| concepts[field].anzsrc:group:3009 | mapping · figshare com | vocabulary-mapper@1.0.0 | keywords['Veterinary sciences'] |
| concepts[field].anzsrc:group:3009 | mapping · repository lboro ac uk | vocabulary-mapper@1.0.0 | keywords['Veterinary sciences'] |
| concepts[field].anzsrc:group:3104 | mapping · repository lboro ac uk | vocabulary-mapper@1.0.0 | keywords['Evolutionary biology'] |
| concepts[field].anzsrc:group:3104 | mapping · figshare com | vocabulary-mapper@1.0.0 | keywords['Evolutionary biology'] |
| concepts[field].anzsrc:group:4202 | mapping · figshare com | vocabulary-mapper@1.0.0 | keywords['Epidemiology'] |
| concepts[field].anzsrc:group:4202 | mapping · repository lboro ac uk | vocabulary-mapper@1.0.0 | keywords['Epidemiology'] |
| concepts[field].anzsrc:group:4206 | mapping · repository lboro ac uk | vocabulary-mapper@1.0.0 | keywords['Public health'] |
| concepts[field].anzsrc:group:4206 | mapping · figshare com | vocabulary-mapper@1.0.0 | keywords['Public health'] |
| concepts[field].local:field:astronomy | mapping · figshare com | connector:figshare_com@1.0.0 | |
| concepts[field].local:field:chemistry | mapping · repository lboro ac uk | connector:repository_lboro_ac_uk@1.0.0 | |
| concepts[field].local:field:chemistry | mapping · figshare com | connector:figshare_com@1.0.0 | |
| concepts[field].local:field:computer-science-ai | mapping · repository lboro ac uk | connector:repository_lboro_ac_uk@1.0.0 | |
| concepts[field].local:field:computer-science-ai | mapping · figshare com | connector:figshare_com@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · figshare com | connector:figshare_com@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · repository lboro ac uk | connector:repository_lboro_ac_uk@1.0.0 | |
| concepts[field].local:field:economics-finance | mapping · repository lboro ac uk | connector:repository_lboro_ac_uk@1.0.0 | |
| concepts[field].local:field:economics-finance | mapping · figshare com | connector:figshare_com@1.0.0 | |
| concepts[field].local:field:engineering | mapping · figshare com | connector:figshare_com@1.0.0 | |
| concepts[field].local:field:engineering | mapping · repository lboro ac uk | connector:repository_lboro_ac_uk@1.0.0 | |
| concepts[field].local:field:humanities | mapping · figshare com | connector:figshare_com@1.0.0 | |
| concepts[field].local:field:humanities | mapping · repository lboro ac uk | connector:repository_lboro_ac_uk@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · repository lboro ac uk | connector:repository_lboro_ac_uk@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · figshare com | connector:figshare_com@1.0.0 | |
| concepts[field].local:field:materials-science | mapping · repository lboro ac uk | connector:repository_lboro_ac_uk@1.0.0 | |
| concepts[field].local:field:mathematics-statistics | mapping · repository lboro ac uk | connector:repository_lboro_ac_uk@1.0.0 | |
| concepts[field].local:field:medicine-health | mapping · repository lboro ac uk | connector:repository_lboro_ac_uk@1.0.0 | |
| concepts[field].local:field:medicine-health | mapping · figshare com | connector:figshare_com@1.0.0 | |
| concepts[field].local:field:ocean-atmospheric | mapping · figshare com | connector:figshare_com@1.0.0 | |
| concepts[field].local:field:psychology-behavioral | mapping · repository lboro ac uk | connector:repository_lboro_ac_uk@1.0.0 | |
| concepts[field].local:field:social-science | mapping · figshare com | connector:figshare_com@1.0.0 | |
| concepts[field].local:field:social-science | mapping · repository lboro ac uk | connector:repository_lboro_ac_uk@1.0.0 | |
| description | source · figshare com | connector:figshare_com@1.0.0 | /metadata/dc/description |
| license | source · figshare com | connector:figshare_com@1.0.0 | /metadata/dc/rights |
| publication_date | source · figshare com | connector:figshare_com@1.0.0 | |
| title | source · figshare com | connector:figshare_com@1.0.0 | /metadata/dc/title |