Omics · study · 2026
Nuclear transcriptome analysis-based verification of sample purity for Purkinje cell nuclei isolated by fluorescence-activated nuclear sorting (FANS).
Listed in NCBI GEO
We employed fluorescence-activated nuclear sorting (FANS) or Purkinje cells from post-mortem brain tissue of individuals carrying an expanded CAG repeat in ATXN3 gene, a mutation causing spinocerebellar ataxia type 3 (SCA3).
Description
Purity of sorted samples was verified by comparing the nuclear transcriptome of Purkinje cell nuclei to the transcriptome of cerebellar granule cell nuclei and glial cell nuclei isolated from unaffected control individuals.
The goal was to assess the stability of ATXN3 CAG repeat in pure Purkinje cell samples.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE327nnn/GSE327324 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE327324 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1450520 ncbi.nlm.nih.gov/bioproject/PRJNA1450520 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Homo sapiens
- From keywords
- Life Sciences
Provenance · 1 source records, 7 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE327324 | 13 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[organism].NCBITaxon:9606 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |