2603 metagenome-assembled genomes (MAGs) from the Bohai Sea and Yellow Sea samples

A total of 24 sampling stations were established across the three transects (Bohai Sea, North Yellow Sea and South Yellow Sea), covering 32.97-39.58 N and 119.01-123.98 E (in decimal degrees). At each station, surface (approximately 3 m) and near-bottom (17-78 m) seawater was collected and filtered through 0.22-um pore-size membranes to enrich microbial biomass, yielding 48 metagenomic samples in total.

All samples were sequenced using a 150-bp paired-end strategy on BGI high-throughput sequencing platforms and quality controlled by fastp. Based on this dataset, we performed per-sample assembly, multi-sample binning based on cross-sample coverage profiles, genome quality assessment, dereplication and quality filtering, constructing a genome catalog comprising 2603 high-quality MAGs for downstream analyses.</p>