Omics · study · 2026
Bulk RNA-seq of human iPSC-derived epithelial cysts and trunk morphs
Listed in NCBI GEO
This series contains bulk RNA-sequencing data from human induced pluripotent stem cell-derived epithelial cysts and trunk morphs generated on microwell chips with localized delivery of BMP4 from coated beads.
Description
Four sets of libraries are included: (1) pluripotent cysts collected 0, 7 and 24 h after exposure to BMP4-coated beads, used to follow induction of endogenous BMP4 expression; (2) cysts after two days of differentiation in the presence of the Activin/Nodal inhibitor A83-01, and cysts differentiated without the inhibitor, used to test whether definitive endoderm is induced under the differentiation conditions used in this study; (3) sorted neuromesodermal progenitor and lateral plate mesoderm populations; and (4) cells carrying a CRISPR interference construct targeting the BMP receptor BMPR1A compared with cells carrying a scrambled guide control.
Together these data characterise the transcriptional response to localized BMP signalling and the specification of lateral plate mesoderm in this model.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE347nnn/GSE347564 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE347564 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1530521 ncbi.nlm.nih.gov/bioproject/PRJNA1530521 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Homo sapiens
- From keywords
- Life Sciences
- Inferred from text
- RNA sequencing 65%
Provenance · 1 source records, 8 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE347564 | 11 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[organism].NCBITaxon:9606 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |