Transcriptomic profiling of UBE2C depletion and overexpression in MCF-7 breast cancer cells

Two independent bulk RNA-sequencing comparisons were included: a CRISPR/Cas9-derived UBE2C-depleted clone and its corresponding sgCtrl clone, and UBE2C-overexpressing cells and their matched vector control. For each condition, total RNA was independently extracted from three separately cultured cell preparations and subsequently pooled after RNA extraction and before library preparation, resulting in one pooled RNA-seq library per condition.

The four pooled libraries therefore represent two independent UBE2C perturbation comparisons rather than independently sequenced biological replicates. The datasets were generated to characterize transcriptional programs associated with loss and gain of UBE2C expression and to support subsequent targeted experimental validation.