Omics · study · 2026
Bulk RNA sequencing of STK11-deficient and parental Lacun3 murine lung adenocarcinoma cells
Listed in NCBI GEO
STK11 mutations are associated with primary resistance to immune checkpoint inhibitors in lung adenocarcinoma, but the tumor cell-intrinsic mechanisms underlying this phenotype remain incompletely understood.
Description
To investigate transcriptional changes induced by STK11 loss independently of the tumor microenvironment, bulk RNA sequencing was performed on parental Lacun3 murine lung adenocarcinoma cells and CRISPR-generated Stk11-deficient Lacun3 cells cultured in vitro.
Differential gene expression analysis identified increased expression of several mediators associated with complement activation and neutrophil extracellular trap (NET) formation, supporting a tumor cell-intrinsic transcriptional program that promotes a pro-NETotic tumor microenvironment.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE338nnn/GSE338923 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE338923 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1496386 ncbi.nlm.nih.gov/bioproject/PRJNA1496386 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
- Inferred from text
- Cancer 65% · RNA sequencing 75% · Sequencing 75%
Provenance · 1 source records, 10 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE338923 | 13 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[disease].local:disease:cancer | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |