Blocking the Gut–Liver IgA Axis Attenuates Macrophage-Mediated Inflammation and Injury in Alcohol-Associated Liver Disease [RNA-Seq]

CD14⁺ monocytes were stimulated on plate-bound IgA (in PBS) for 24 hours or cultured on mock-coated plates incubated with PBS alone. Libraries were prepared using the QuantSeq 3′ mRNA-Seq V2 Library Prep Kit FWD (Lexogen) and sequenced as single-end 100 bp reads on an Illumina NextSeq 2000.

This series includes three technical replicates per condition from a single donor.