Bulk RNA sequencing study of iGCB day3 cells to investigate differences between Eif3e cg1cre knockout B cells and control B cells

By day 3 of culture, we harvested abundant YFP+ Eif32-deficient B cells and their corresponding control cells. RNA-seq comparison revealed that Eif3e-deficient B cells upregulated some co-stimulatory molecules, including Cd80 and Icam1.

GO enrichment analysis showed that upregulated genes were primarily associated with ribosome-related pathways, while downregulated genes were mainly enriched in lipid metabolism. The upregulation of co-stimulatory molecules may confer Eif3e-deficient B cells with the capacity to promote CD4+ T cell activation.