WTAP tetramer ensures m⁶A writer assembly and faithful mitosis

Wilms’ tumor 1-associating protein (WTAP) is a conserved regulatory subunit essential for m⁶A deposition and cell proliferation, yet its structural organization and mechanistic contributions remain elusive.Our live-cell imaging demonstrates that WTAP is required for accurate chromosome segregation, and MeRIP-seq analysis identifies WTAP-dependent m⁶A regulation as a critical determinant sustaining the expression of mitotic regulators, including KIF20A.

Together, our study defines a tetrameric scaffold function for WTAP that is essential for writer complex integrity and highlights its pivotal role in linking m⁶A methylation to cell cycle progression.