Transcriptional profiles upon MNDA overexpression in iPSC-derived human Sertoli-like cells

To explore the regulatory roles of MNDA in Sertoli cell physiological function, we used human induced pluripotent stem cell-derived Sertoli-like cells (hiSCs) as an in vitro human Sertoli cell model, whose preparation procedures were adapted from the previously reported study (Liang et al., eLife, 2019). MNDA was overexpressed in hiSCs through lentiviral transduction, followed by RNA-seq profiling and bioinformatic analysis.

The results showed that MNDA overexpression led to downregulation of genes enriched in glycoprotein biosynthesis, cell-matrix adhesion and basal plasma membrane, accompanied by upregulation of genes involved in rhythmic process and actin filament bundle organization. Collectively, these data revealed the effects of MNDA on Sertoli cell function at the transcriptional level.