High-throughput screen identifies metabolite regulators of endothelial function

Metabolites are critical regulators of EC health and function, yet only a limited number of metabolites that impact ECs have been identified. Here, we undertook a high-throughput screen of 884 metabolites and common drugs found in human to systematically identify metabolites that impact barrier function of primary human umbilical cord vein ECs (HUVECs).

Seven metabolites that deregulate vascular barrier function without causing cell death were shortlisted. This includes (-)-epigallocatechin gallate, (±)-3-methyl-2-oxovaleric acid, taurocholic acid, myricetin, meloxicam, hesperetin and trans-3-indoleacrylic acid. These metabolites elicited organ-specific responses, and showed differential effects on coronary artery ECs, lung ECs and HUVECs.