Three immunoregulatory signatures define non-productive HIV infection in stem cell memory CD4+ T cells

Our understanding of how non-productive HIV infections are established in primary human CD4+ T cells during the first round of infection is, however, still incomplete. In this study, we leverage the HIV reporter virus pMorpheus-V5 to delineate cellular expression patterns that are upregulated in non-productively infected primary stem cell memory (TSCM) CD4+ T cells.

We find that CD4+ TSCM harboring non-productive proviruses display a distinct transcriptomic signature comprising 118 upregulated genes. This non-productive expression profile is distinct from that of productively infected cells as well as from negative-exposed and mock-infected cells. Among the cellular genes most upregulated in CD4+ T cells harboring non-productive proviruses are CCR4-binding migratory chemokines (CCL22, CCL17), tryptophan catabolic enzymes (IDO1, KYNU), and genes encoding cytoskeletal rearrangement proteins (BASP1, TNFAIP2).