Omics · study · 2026
Reduced Representation Bisulfite Sequencing of Mouse Hearts Following Cardiomyocyte-Specific DNMT3A Overexpression
Listed in NCBI GEO
Aging is associated with extensive epigenetic remodeling in cardiomyocytes, including increased DNA methylation.
Description
To investigate the causal role of DNA hypermethylation in age-related cardiac dysfunction, we overexpressed DNA methyltransferase 3A (DNMT3A) specifically in cardiomyocytes of young adult mice using a cardiac-specific AAV vector. Reduced representation bisulfite sequencing (RRBS) was performed on isolated cardiomyocyte nuclei from DNMT3A-overexpressing (Dnmt3a OE) and control (GFP) hearts.
This dataset provides genome-wide DNA methylation profiles for both groups, enabling analysis of DNMT3A-driven methylome remodeling in the heart.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE299nnn/GSE299673 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE299673 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1275948 ncbi.nlm.nih.gov/bioproject/PRJNA1275948 ↗
project · from NCBI GEO
Topics
- Stated by source
- Methylation profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
- Inferred from text
- Heart 75% · Sequencing 75%
Provenance · 1 source records, 9 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE299673 | 14 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[anatomy].local:anatomy:heart | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:methylation-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |