Chemokine receptor expression patterns delineate distinct interstitial macrophage subsets in the lung and define a trajectory from monocytes to mature macrophages, bulk RNA-sequencing data

However, the discrete role for each receptor in monocyte/macrophage biology remains poorly understood, with previous reports citing receptor redundancy. Here, we have used transcriptomic approaches to examine inflammatory chemokine receptor expression on lung interstitial macrophage populations.

We have demonstrated that interstitial macrophages can be divided into three distinct subsets, each of which express specific patterns of chemokine receptors, and that there are dynamic changes in chemokine receptor expression as macrophages differentiate from monocytes in the lung. Furthermore, macrophages expressing different combinations of chemokine receptors are transcriptionally distinct from one another, suggesting non-redundant functions for CCR 1, 2 and 5.