Tumor suppressor genotype influences the extent and mode of immunosurveillance in lung cancer

To better understand the contribution of tumor genotype to immunosurveillance, We generated and validated lentiviral vectors that establish an epi-allelic series of increasingly immunogenic neoantigens, integrating them with autochthonous Cre-regulated cancer models, CRISPR/Cas9-mediated somatic genome editing, and tumor barcoding into a single system.

Using this sytem, we show that in the context of KRAS-driven lung cancer and strong neoantigen expression, tumor suppressor genotype dictates the degree of immune cell recruitment, positive selection of tumors with neoantigen silencing, and tumor outgrowth.