Integrated Multi-omics Profiling of Mouse Brain Tissue Using the AllPrep Methods versus Traditional Extraction Methods

At the transcriptomic level, AllPrep produced higher RNA yields and an 88.14% gene detection rate, ensuring broader coverage. In proteomics, it preserved identification depth while improving detection of membrane proteins, ubiquitin ligases, and transcription factors.

Phosphoproteomics further revealed 4,347 high-confidence phosphorylation sites, including CAMK family regulatory patterns. Integrated analysis showed stronger RNA–protein correlations and enriched neuroscience-related pathways. Overall, AllPrep provides a standardized, scalable workflow that enhances data quality and supports deeper exploration of neurobiological mechanisms.