Omics · study · 2026
A Fibroblast-Mediated Desmoplastic Reaction Restrains Gastric Cancer via YAP-CTRB1-driven Competitive Fitness
Listed in NCBI GEO
Purpose: To explore the molecular mechanism of CTRB1 boosts host endows cell fitness to outcompete tumor cells, RNA sequencing was performed to analyzeand compare the gene signatures of between MFCSTCL interacted L929 cells (Biotin+) and tumor uninteracted L929 cells (Biotin-).
Description
Methods
Tumor interacted or uninteracted L929 cells were sorted. Total mRNA was extracted from tumor interacted (Biotin+) or uninteracted (Biotin-) L929 cells. Then RNA quality was assessed using an Agilent Bioanalyzer 2100 and the sample reads were sequenced using Illumina NovaSeq 6000 platform.
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Results: Our results revealed differentially expressed genes in Biotin+ L929 cells when compared with Biotin- L929 cells. Further KEGG analysis reveal that expression of CTRB1 influenced several proliferation-related signaling pathways, including Hippo-YAP, WNT, and Notch. Conclusion: Our study present the detailed transcripts analysis of tumor interacted (Biotin+) or uninteracted (Biotin-) L929 cells.
Based on RNA-seq transcriptome characterization, indicating that CTRB1 can activate multiple proliferation-related signaling pathways expression to dictate cell fitness during cell competition.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE332nnn/GSE332729 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE332729 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1468993 ncbi.nlm.nih.gov/bioproject/PRJNA1468993 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
- Inferred from text
- Cancer 75% · RNA sequencing 75% · Sequencing 75%
Provenance · 1 source records, 10 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE332729 | 10 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[disease].local:disease:cancer | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |