Omics · study · 2026
RNA-seq profiling of TGF-beta induction and TXNRD1 loss in A549 lung cancer cells.
Listed in NCBI GEO
We performed bulk RNA-seq to investigate transcriptional responses to TGF-β1 signaling in A549 human lung adenocarcinoma cells with different TXNRD1 genetic backgrounds.
Description
Wild-type A549 cells and TXNRD1-knockdown A549 cells generated by single-guide RNA targeting TXNRD1 (sgTXNRD1) were analyzed under basal conditions and following TGF-β1 induction. This dataset aims to elucidate the role of TXNRD1 in modulating TGF-β1–driven transcriptional programs in lung cancer cells.
Total RNA was extracted from cultured A549 cells, and sequencing libraries were prepared using a standard RNA-seq library construction protocol. Libraries were sequenced on an Illumina platform to generate high-quality reads for transcriptome profiling. Reads were aligned to the human reference genome (GRCh38) using STAR (v2.7.10a), and gene-level expression was quantified with featureCounts (v2.0.3) based on GENCODE annotation (v38).
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Raw sequencing data and processed gene-level count matrices are provided to support downstream analyses and data reuse.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE316nnn/GSE316125 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE316125 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1401575 ncbi.nlm.nih.gov/bioproject/PRJNA1401575 ↗
project · from NCBI GEO
- PubMed 42709540 pubmed.ncbi.nlm.nih.gov/42709540 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Homo sapiens
- From keywords
- Life Sciences
- Inferred from text
- Cancer 75% · RNA sequencing 65% · Sequencing 75%
Provenance · 1 source records, 10 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE316125 | 12 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[disease].local:disease:cancer | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:9606 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |