Data Sheet 1_CircUGP2 inhibits tumor progression via PI3K/AKT/mTOR signaling through sponging miR-137-3p and interacting with UPF1 in hepatocellular carcinoma.xlsx

Subsequently, an assessment was conducted to determine the biological effects of circUGP2, employing a series of analytical methods, including CCK8, EdU, colony formation, transwell, scratch assays, and in vivo experimentation.

To explore the functions of circUGP2 as a sponge, including RNA pull-down, microRNA-seq, fluorescence in situ hybridization (FISH), RNA immunoprecipitation (RIP), and luciferase reporter assays were employed. Mass spectrometry (MS), RIP, and co-inmunoprecipitation (Co-IP) were employed to examine the interaction between cirrcUGP2 and up-frameshift protein 1 (UPF1).</p>Results<p>circUGP2 was downregulated in HCC tissues and cells, which inhibited the growth and spread of HCC in vitro and in vivo.