Processed single-cell RNA-seq data and analysis code for characterizing microglial PTM enzyme modules in cerebral ischemia-reperfusion injury

The study identifies three PTM enzyme co-expression modules (M1/M2/M3) in microglia after cerebral ischemia-reperfusion injury and characterizes the functional coupling between the M2 module, histone lactylation, and ferroptosis/PANoptosis gene programs.Contents:seu_mg_scored.rds: Processed Seurat object containing 10,275 high-confidence microglia with all calculated module scores (M2, LRG, FRG, PRG).

This is the primary object used for all single-cell analyses in the study.Validation_datasets/: A directory containing processed expression matrices and Seurat objects for the independent validation datasets (GSE131193, GSE240368, GSE272754, GSE283233).Code/: R scripts used to perform all analyses, generate figures, and calculate statistics. The README.md file within describes the required R environment and dependencies.Figures/: High-resolution PDF versions of all main and supplementary figures presented in the manuscript.Data Generation and Processing: The primary scRNA-seq dataset (GSE174574) was downloaded from GEO.