Raw data of "Single-cell RNA sequencing analysis revealed a novel porcine alveolar macrophage subtype with high SLMAF7 expression promoting PRRSV infection"

Interestingly, bystander cells in displayed similar inflammatory responses to those in PRRSV-infected cells, suggesting a role for bystander cells in the inflammatory response. Through scRNA-seq analysis, transcriptomic profiling of cells pre- and post-PRRSV infection revealed several distinct subclusters (S1–S8 and SS1–SS7), with the S6 subpopulation exhibiting the highest PRRSV infected rate and the SS7 subpopulation showing the fastest PRRSV replication rate.

The SLMAF7 emerged as a key gene in these two subpopulations for PRRSV infection. Knockdown of SLMAF7 significantly reduced PRRSV infection and suppressed PRRSV-induced inflammation. Collectively, our findings provide insights into the host response to PRRSV infection and identify SLAMF7 as a novel potential therapeutic target tackling PRRSV infection.