Omics · study · 2026
APOE3 and APOE4 Human Astrocytes Differentially Modulate Alzheimer’s Disease Pathology and Microglial Responses in Chimeric Mice
Listed in NCBI GEO
To investigate how APOE3 and APOE4 human iPSC-derived astrocytes influence the gene expression profile of the mouse host microglia in vivo, we performed bulk RNA sequencing of cortical regions adjacent to transplanted astrocytes in AD chimeric mice.
Description
Human iPSC-derived astrocytes (APOE3 or APOE4) were transplanted into neonatal Alzheimer's disease model mice and analyzed 6-7 months post-transplantation. Mouse-specific transcripts were analyzed to compare the transcriptomic profiles of host microglia in AD chimeric mice engrafted with APOE4 versus APOE3 human astrocytes.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE335nnn/GSE335500 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE335500 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1478654 ncbi.nlm.nih.gov/bioproject/PRJNA1478654 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
- Inferred from text
- Disease 75% · RNA sequencing 75% · Sequencing 75%
Provenance · 1 source records, 10 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE335500 | 12 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[disease].local:disease:disease | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |