20 datasets

NCBI GEO2026 · RNA sequencing · 6 samples

…This transformation requires precise spatial and temporal coordination among diverse cardiac cell types; central to this process is cardiomyocyte maturation, yet the regulatory mechanisms… …Here, we present a temporal and spatial atlas of postnatal hearts by integrating single-nucleus transcriptomics with image-based spatial transcriptomics, which uncovers the dynamic…

NCBI GEO2026 · RNA sequencing · 40 samples

…Here, we established in vivo single-cell RNA metabolic labeling in the adult heart to systematically identify the cells that first respond to injury, as well as the processes that are… …In conjunction with detailed dissection of single-cell transcriptomics atlases, this finding led us to postulate that macrophage-specific inhibition of Toll-like receptor signaling…

NCBI GEO2026 · RNA sequencing · 294 samples

…This study aimed to clarify the gene expression of each of cardiac neural crest cells during mouse heart development by using Fluidigm C1 system.… …Embryonic hearts from Wnt1-Cre; R26-EYFP mice, which labeled neural crest cells, at E11.5, E12.5, E14.5 and E17.5 were used for single-cell (sc) RNA-seq.…

NCBI GEO2026 · RNA sequencing · 12 samples

…Single-nucleus RNA sequencing (snRNA-Seq) enables high-resolution transcriptomic profiling of complex tissues such as the heart, where dissociation into intact single cells is challenging… …Both platforms resolved major cardiac cell populations with consistent cell-type proportions and marker gene expression.…

NCBI GEO2026 · RNA sequencing · 11 samples

…interspecies issues of animal models and oversimplicity of single cell type two-dimensional cell models to recapitulate cardiac (patho)physiology.… …It acts as a source of progenitor cells, thus contributing to cardiac fibroblast (CF), vascular smooth muscle cell (VSMC) and pericyte populations in heart, and participates in essential…

NCBI GEO2026 · RNA sequencing · 2 samples

…To identify cells that physically interact with vascular endothelial cells in vivo, we utilized the REFLEX system combined with HUNTERuni-seq, a single-cell transcriptomic approach… …Cdh5-CreERT2;REFLEX mice were subjected to transverse aortic constriction (TAC), and GFP-positive cells representing endothelial-interacting cells were isolated from the heart.…

NCBI GEO2026 · Methylation profiling by genome tiling array · 8 samples

…interspecies issues of animal models and oversimplicity of single cell type two-dimensional cell models to recapitulate cardiac (patho)physiology.… …It acts as a source of progenitor cells, thus contributing to cardiac fibroblast (CF), vascular smooth muscle cell (VSMC) and pericyte populations in heart, and participates in essential…

NCBI GEO2026 · RNA sequencing · 12 samples

…We show in this study that endothelial Tie1 and Tek are highly expressed in endocardial cells of atria by the single cell RNA-seq analysis.… …The bulk RNA-seq analysis of hearts at the four-chambered stage revealed that gene transcripts related to endothelial cell development and cardiac trabeculation were reduced in the…

NCBI GEO2026 · RNA sequencing · 12 samples

…We carried out single-nucleus RNA sequencing (snRNA-seq) on 11 non-failing and 8 ARVC patient right ventricular samples and transcriptionally profiled 139,347 nuclei.… …We identified over 4,400 differentially expressed protein coding genes between non-failing and ARVC samples across cell types.…

NCBI GEO2026 · RNA sequencing · 4 samples

…Vascular smooth muscle cells (VSMCs) in the ascending aorta and aortic arch arise from two distinct embryonic origins: the second heart field (SHF) and the cardiac neural crest (CNC… …The ascending aorta and aortic arch were dissected from male and female mice, and single-cell suspensions were generated by enzymatic digestion.…

NCBI GEO2026 · RNA sequencing · 8 samples

…Our study directly addresses this critical knowledge gap by performing an integrative multi-omics study—combining single-nucleus RNA/ATAC sequencing and high-resolution spatial transcriptomics… …(MERFISH) on human EFE and healthy heart tissues.…

NCBI GEO2026 · RNA sequencing · 37 samples

…Single-cell RNA sequencing was employed to analyze transcriptional changes in cardiac immune cells.… …Results: Circulating soluble CD163 levels were markedly elevated in patients with ICM-induced heart failure compared with individuals without heart failure (median difference 34.5 ng…

NCBI GEO2026 · RNA sequencing · 22 samples

…Spatial mapping revealed aberrant interactions between these cardiomyocytes, fibroblasts, and immune cells, contributing to tissue-wide transcriptional changes in cKO hearts.… …These findings suggest that NE rupture in cKO cardiomyocytes triggers cytosolic DNA sensing pathways and maladaptive cell-cell communication with fibroblasts and immune cells, leading…

NCBI GEO2026 · RNA sequencing · 46 samples

…Spatial mapping revealed aberrant interactions between these cardiomyocytes, fibroblasts, and immune cells, contributing to tissue-wide transcriptional changes in cKO hearts.… …These findings suggest that NE rupture in cKO cardiomyocytes triggers cytosolic DNA sensing pathways and maladaptive cell-cell communication with fibroblasts and immune cells, leading…

NCBI GEO2026 · RNA sequencing · 6 samples

…A key contributing factor is the Patients with single ventricle congenital heart defects (SVCHDs) urgently require TEVGs for cardiopulmonary reconstruction during Fontan procedure.… …left heart syndrome (HLHS), one severe form of SVCHD.…

NCBI GEO2026 · RNA sequencing · 6 samples

…The heart is one of the most energy-demanding organs in the body, and mitochondria are the main places where energy is produced within cells.… …In addition, single-cell RNA-sequencing (scRNA-seq) data (GSE174458) was employed to reveal cellular composition in myocarditis.…

NCBI GEO2026 · RNA sequencing · 9 samples

…) cells.… …Single-cell RNA-sequencing revealed that Lnαnv-induced PC-blastoids were transcriptionally and metabolically similar to natural blastocysts.…

NCBI GEO2026 · RNA sequencing · 6 samples

…Furthermore, we generated TRIM28 and Hey2 (hes-related family bHLH transcription factor with YRPW motif 2) whole-body single and double heterozygous knockout mice to evaluate their… …In contrast, TRIM28 in the compact layer cooperated with transcription repressor Hey2 to suppress the expression of cell cycle inhibitor P21Cip1/Waf1 (Cyclin-Dependent Kinase Inhibitor…

NCBI GEO2026 · RNA sequencing · 8 samples

…We show in this study that endothelial Tie1 and Tek are highly expressed in endocardial cells of atria by the single cell RNA-seq analysis.… …The bulk RNA-seq analysis of hearts at the four-chambered stage revealed that gene transcripts related to endothelial cell development and cardiac trabeculation were reduced in the…

NCBI GEO2026 · RNA sequencing · 54 samples

…This study investigated the transcriptional effects of gut microbiota depletion and restoration in mice across six tissues (colon, jejunum, liver, heart, lung, and kidney) using whole-transcriptome… …Single-cell data analysis highlighted a potential gut-liver axis interaction, primarily mediated by colonic enterocytes and hepatic cholangiocytes, meanwhile gut microbiota repressed…