Single-cell transcriptomic analysis of GFP-positive and GFP-negative CD45-positive and CD45-negative cardiac cells isolated from TAC-operated REFLEX mice

To identify cells that physically interact with vascular endothelial cells in vivo, we utilized the REFLEX system combined with HUNTERuni-seq, a single-cell transcriptomic approach for profiling interacting cell populations. Cdh5-CreERT2;REFLEX mice were subjected to transverse aortic constriction (TAC), and GFP-positive cells representing endothelial-interacting cells were isolated from the heart.

Single-cell RNA sequencing was performed to characterize the cellular composition and transcriptional states of interacting and non-interacting cell populations in the pressure-overloaded myocardium. These data provide a resource for investigating endothelial-centered cellular interaction networks during cardiac remodeling.