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}NCBI GEO2026 · RNA sequencing · 39 samples
Unravelling Zebrafish Heart Regeneration at Single-Cell Level…Mammals exhibit limited heart regeneration ability, which can lead to heart failure after myocardial infarction.… …We collected major cardiac cell types from zebrafish and performed high-precision single-cell transcriptome analyses during development and post-injury regeneration.…
NCBI GEO2026 · RNA sequencing · 2 samples
Single-Cell Analysis of Changes in the Liver after Murine Heart Transplantation…To accurately illustrate the Changes in the Liver during heart allograft rejection, we established the murine heterotopic heart transplantation model and harvested liver of recipients… …for single cell transcriptome sequencing.…
NCBI GEO2026 · RNA sequencing · 6 samples
Charting Postnatal Heart Development Using In Vivo Single-Cell Functional Genomic…This transformation requires precise spatial and temporal coordination among diverse cardiac cell types; central to this process is cardiomyocyte maturation, yet the regulatory mechanisms… …Here, we present a temporal and spatial atlas of postnatal hearts by integrating single-nucleus transcriptomics with image-based spatial transcriptomics, which uncovers the dynamic…
NCBI GEO2026 · RNA sequencing · 9 samples
Single-cell transcriptomic profiling of the developing zebrafish heart.…We profiled over 34,000 cells from resected zebrafish hearts spanning 10 developmental stages from 25 hours to 60 days post-fertilization.… …This zebrafish heart atlas enhances our understanding of vertebrate heart development and represents a valuable resource for future studies.…
NCBI GEO2026 · RNA sequencing · 90 samples
Single-cell multiomic integration identifies widespread, cell-type resolved fetal reactivation in the diseased human heart…Results: We generate an integrated multiomic atlas of human cardiac cells, combining newly generated and publicly available single-nucleus RNA sequencing datasets from 299 donors and… …single nucleus ATAC-seq datasets from 106 donors.…
NCBI GEO2026 · RNA sequencing · 8 samples
Charting Postnatal Heart Development Using In Vivo Single-Cell Functional Genomic [snRNA-Seq]…This transformation requires precise spatial and temporal coordination among diverse cardiac cell types; central to this process is cardiomyocyte maturation, yet the regulatory mechanisms… …Here, we present a temporal and spatial atlas of postnatal hearts by integrating single-nucleus transcriptomics with image-based spatial transcriptomics, which uncovers the dynamic…
NCBI GEO2026 · RNA sequencing · 3 samples
Charting Postnatal Heart Development Using In Vivo Single-Cell Functional Genomic [PIP-Seq]…This transformation requires precise spatial and temporal coordination among diverse cardiac cell types; central to this process is cardiomyocyte maturation, yet the regulatory mechanisms… …Here, we present a temporal and spatial atlas of postnatal hearts by integrating single-nucleus transcriptomics with image-based spatial transcriptomics, which uncovers the dynamic…
NCBI GEO2026 · RNA sequencing · 2 samples
Single-cell RNA Sequencing of T Cells from Anti-IgG and Anti-PD1 injection Mouse hearts after MI…To determine the activation of PD1+ T cells in MI, we sorted and sequenced PD1+ T cells accompanied with PD1- T cells in the mice heart after MI.… …In order to deeply investigate the function of PD1+ T cells in heart during MI, we performed single-cell RNA sequencing (scRNA-seq) to detect the change T cells from anti-IgG and anti-PD1…
NCBI GEO2026 · RNA sequencing · 3 samples
Identifying transcription factors distinguishing regenerating cardiomyocytes in the zebrafish heart using single cell transcriptomics…Understanding which factors contribute for zebrafish heart regeneration is crucial for developing regeneration-inducing strategies as mammalian hearts are limited in regenerative capacity… …Exploring further our recently published optimized approach for increasing the single cell RNA sequencing (scRNA-seq) resolution specifically CMs, we have systematically evaluated transcription…
NCBI GEO2026 · RNA sequencing · 40 samples
In vivo single-cell RNA metabolic labeling resolves early transcriptional responders in the regenerating zebrafish heart [bulk_slamseq]…Here, we established in vivo single-cell RNA metabolic labeling in the adult heart to systematically identify the cells that first respond to injury, as well as the processes that are… …In conjunction with detailed dissection of single-cell transcriptomics atlases, this finding led us to postulate that macrophage-specific inhibition of Toll-like receptor signaling…
NCBI GEO2026 · RNA sequencing · 4 samples
In vivo single-cell RNA metabolic labeling resolves early transcriptional responders in the regenerating zebrafish heart [scslam-seq]…Here, we established in vivo single-cell RNA metabolic labeling in the adult heart to systematically identify the cells that first respond to injury, as well as the processes that are… …In conjunction with detailed dissection of single-cell transcriptomics atlases, this finding led us to postulate that macrophage-specific inhibition of Toll-like receptor signaling…
NCBI GEO2026 · RNA sequencing · 15 samples
In vivo single-cell RNA metabolic labeling resolves early transcriptional responders in the regenerating zebrafish heart [scrna-seq]…Here, we established in vivo single-cell RNA metabolic labeling in the adult heart to systematically identify the cells that first respond to injury, as well as the processes that are… …In conjunction with detailed dissection of single-cell transcriptomics atlases, this finding led us to postulate that macrophage-specific inhibition of Toll-like receptor signaling…
NCBI GEO2026 · RNA sequencing · 2 samples
Elongating heart organoids recapitulate early heart morphogenesis and model congenital heart disease…While pluripotent stem cell-derived heart organoids model cardiomyocyte differentiation, they do not recapitulate early morphogenetic events.… …Single-cell transcriptomics and trajectory analyses, together with pulse-labeling, support a model in which progressive incorporation of cardiac cells from proliferative splanchnic…
NCBI GEO2026 · RNA sequencing · 294 samples
Gene expression profiles of cardiac neural crest cells during heart development…This study aimed to clarify the gene expression of each of cardiac neural crest cells during mouse heart development by using Fluidigm C1 system.… …Embryonic hearts from Wnt1-Cre; R26-EYFP mice, which labeled neural crest cells, at E11.5, E12.5, E14.5 and E17.5 were used for single-cell (sc) RNA-seq.…
NCBI GEO2026 · RNA sequencing · 6 samples
A Cost-Effective Nuclei Isolation Protocol for Single Nucleus RNA-Sequencing of Mouse HFpEF Hearts…Single cell or single nuclei RNA-sequencing (snRNAseq) enables high-resolution profiling of cellular states, but sample preparation is often costly and complicated by debris formation… …and cell clumping.…
NCBI GEO2026 · RNA sequencing · 12 samples
Absence of a prolonged macrophage and B cell response inhibits heart regeneration in the Mexican cavefish…The Mexican cavefish provides a unique comparative model to study heart regeneration and scarring within a single species, with Surface fish capable of heart regeneration and their… …Using single-cell transcriptomics and immune perturbations, we find significant differences in the immune response between the two populations.…
NCBI GEO2026 · Excel spreadsheet · 6 samples
Bulk RNA-seq of PD1+/- T cels from mouse hearts 7 days post MI…In order to deeply investigate the expression characteristics of PD1+ T cells in heart during myocardial infarction (MI), we firstly performed single-cell RNA sequencing (scRNA-seq)… …In order to deeply investigate the function of PD1+ T cells in heart during MI, we performed single-cell RNA sequencing (scRNA-seq) to detect the change T cells from anti-IgG and anti-PD1…
NCBI GEO2026 · RNA sequencing · 4 samples
Single-cell RNA Sequencing of non-cardiomyocytes from mouse after MI…Programmed cell death protein 1 (PD1) is crucial for adaptive immunity regulation, showing considerable effects in various diseases especially in tumor field.… …In order to deeply investigate the expression characteristics of PD1+ T cells in heart during myocardial infarction (MI), we firstly performed single-cell RNA sequencing (scRNA-seq)…
NCBI GEO2026 · RNA sequencing · 2 samples
Recovery from Heart Failure: Microvascular Mechanisms…Background: Heart failure (HF) is a significant global health problem.… …Methods: Myocardial tissues were obtained from patients with HF at the time of LVAD implantation (pre-LVAD) and explantation (post-LVAD) for histological analysis and single-nucleus…
NCBI GEO2026 · RNA sequencing · 8 samples
Hypoxia differentially affects coronary vessel formation during heart development…Changes in coronary ECs in these hearts relative to littermate controls were assessed by single cell RNA-sequencing.… …This identified increased angiogenic gene expression and a shift towards a non-proliferative, migratory tip cell phenotype.…
NCBI GEO2026 · RNA sequencing · 14 samples
Transcriptomic analysis of embryonic hearts from the Dp1Tyb mouse model of Down syndrome using scRNAseq…We performed single cell RNAseq on whole hearts from E13.5 Dp1Tyb embryos and from wild-type littermates.…
NCBI GEO2026 · RNA sequencing · 1 samples
A single-cell RNA sequencing dataset of cardiac aging in African Turquoise Killifish…Alterations across different cardiac cell types must be tightly orchestrated during normative aging process that has begun to be mapped at the transcriptional level through single-cell… …Despite its experimental advantages, single-cell transcriptomic studies on cardiac aging in ATK have not yet been conducted.…
NCBI GEO2026 · RNA sequencing · 3 samples
Sustained αβ T Cell Effector Activity and Memory Expansion in Children with Congenital Heart Disease…Congenital heart disease (CHD) is a major global health problem. Although treatment has improved, children still face comorbidities that may shorten their life.… …Single-cell transcriptomics and functional assays revealed high activation states of T cells and higher granzyme and effector cytokine production in children with CHD.…
NCBI GEO2026 · RNA sequencing · 2 samples
Adm and Ackr3 regulate cardiac lymphatic vessel formation during zebrafish heart development and regeneration…It is well established that lymphatic vessels regulate interstitial fluid homeostasis and facilitate the transport of macromolecules and immune cells.… …Single-cell RNA sequencing revealed that, during heart regeneration, ackr3a mutants exhibit altered LEC subcluster compositions and differential expression of several candidate downstream…
NCBI GEO2026 · RNA sequencing · 4 samples
Single-nucleus RNA sequencing of mouse hearts after pressure overload-induced heart failure…Pressure overload_induced heart failure is associated with extensive cellular remodeling in the heart.… …To investigate the cell type_specific transcriptional responses regulated by miR_210, single_nucleus RNA sequencing (snRNA_seq) was performed on heart tissues collected from wild_type…