Omics · study · 2026
Effect of Ttyh2 Knockout on Tumor Growth Control [scRNA-Seq]
Listed in NCBI GEO
We employed single-cell RNA sequencing (scRNA-seq) using the 10x Genomics platform to investigate the role of Ttyh2 in tumor growth control.
Description
Ttyh2 knockout (KO) mice exhibited impaired tumor growth. In wild-type (WT) mice, Ttyh2 expression was enriched in Cd206⁺ tumor-associated macrophages (TAMs) and a subset of dendritic cells (DCs).
In Ttyh2 KO tumors, the Nos2⁺/Cd206⁺ TAM ratio was elevated, accompanied by a compensatory expansion of monocytes. Pathway analysis revealed upregulation of type I interferon and NF-κB signaling signatures in both TAM subsets and cDC1 cells. Additionally, Ttyh2 KO tumors displayed increased infiltration of CD8⁺ T cells and a reduction in regulatory T cells (Tregs).
Read the rest (1 more)
These findings suggest that Ttyh2 deficiency remodels both the myeloid and lymphoid compartments toward a pro-inflammatory, antitumor immune state.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE307nnn/GSE307906 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE307906 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1327778 ncbi.nlm.nih.gov/bioproject/PRJNA1327778 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
- Inferred from text
- Cancer 65% · RNA sequencing 75% · Sequencing 75% · Single-cell RNA sequencing 75%
Provenance · 1 source records, 11 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE307906 | 14 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[disease].local:disease:cancer | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:single-cell-rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |