Inflammatory memory primes the intestine for enhanced regenerative potential [multiome 2]

To investigate whether this dedifferentiation process leads to lasting transcriptomic and epigenetic changes, we used Krt20CreERT2:Rosa26-tdTomato mice to specifically label villus epithelial cells. In non-irradiated conditions, these cells are rapidly shed and lost.

However, following irradiation, a subset of labeled cells dedifferentiates, migrates into the crypt compartment, and contributes to ISC regeneration. To characterize long-term molecular changes in these dedifferentiated cells and their progeny, we performed single-cell multiome sequencing (scRNA-seq and scATAC-seq) using the 10x Genomics Chromium platform.