Omics · study · 2026
Single-nucleus RNA-seq analysis of whole brains from 5xFAD mice with microglia-specific CD31 knockdown
Listed in NCBI GEO
Description
We performed single-nucleus RNA sequencing (snRNA-seq) on whole-brain tissues from 5xFAD mice with microglia-specific CD31 knockdown to investigate transcriptional alterations associated with Alzheimer’s disease pathology. snRNA-seq analysis identified cell-type-specific transcriptional changes across multiple brain cell populations, including microglia, revealing that CD31 knockdown attenuated disease-associated microglial signatures and inflammatory responses while promoting pathways related to Aβ clearance.
These findings provide insights into the role of microglial CD31 in regulating neuroinflammation and Alzheimer’s disease progression.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE331nnn/GSE331294 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE331294 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1467618 ncbi.nlm.nih.gov/bioproject/PRJNA1467618 ↗
project · from NCBI GEO
- PubMed 42248891 pubmed.ncbi.nlm.nih.gov/42248891 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
- Inferred from text
- Biochemistry and cell biology 70% · Disease 75% · RNA sequencing 75% · Sequencing 75% · Single-cell RNA sequencing 65%
Provenance · 1 source records, 12 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE331294 | 14 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[disease].local:disease:disease | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].anzsrc:group:3101 | enrichment · NCBI GEO | taxonomy-embedding@1.1.0 | title+keywords+description (70%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:single-cell-rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |