Omics · study · 2026
Single-cell transcriptomic profiling of NRasG12V/myrAKT-driven hepatocellular carcinoma with Sepp1 knockdown and selenium supplementation
Listed in NCBI GEO
The immune microenvironment of hepatocellular carcinoma (HCC) is strongly influenced by tumor-infiltrating neutrophils, whose functional heterogeneity and regulatory mechanisms remain incompletely defined.
Description
Selenium metabolism, mediated by selenoprotein P (Sepp1), plays an important role in redox homeostasis and immune regulation in the liver.</p><p>In this study, Ras/AKT-driven murine HCC models with Sepp1 knockdown were generated under control or selenium-supplemented conditions.
Single-cell RNA sequencing was performed using the 10x Genomics Chromium platform to profile tumor tissues and the associated tumor microenvironment, with a particular focus on neutrophil populations.</p><p>This dataset enables systematic analysis of neutrophil transcriptional states associated with selenium metabolism and immunoregulatory pathways in HCC. The data provide a resource for investigating how alterations in Sepp1 expression and selenium availability shape tumor-immune interactions at single-cell resolution.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE317nnn/GSE317333 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE317333 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1405944 ncbi.nlm.nih.gov/bioproject/PRJNA1405944 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
- Inferred from text
- Cancer 65% · RNA sequencing 75% · Sequencing 75% · Single-cell RNA sequencing 75%
Provenance · 1 source records, 11 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE317333 | 14 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[disease].local:disease:cancer | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:single-cell-rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |