Omics · study · 2026
Single-cell RNA sequencing reveals Reg3g-dependent alteration of alveolar macrophage transcriptomes during LPS-induced acute lung injury
Listed in NCBI GEO
To investigate how Reg3g modulates alveolar macrophage (AM) transcriptional programs during acute lung injury, we performed single-cell RNA sequencing on fluorescence-activated cell sorting (FACS)-purified CD45⁺Ly6G⁻F4/80⁺CD11b/c⁺ lung macrophages from mice treated with PBS, LPS, or LPS + Reg3g for 24 hours.
Description
Libraries were generated using the 10x Genomics Chromium GEM-X Single Cell 3′ platform. Following quality control, approximately 29,000 cells were retained.
Unsupervised clustering identified 22 distinct cell populations. Reclustering of AMs revealed multiple transcriptional states. LPS exposure shifted AMs toward a pro-inflammatory phenotype, whereas Reg3g treatment attenuated this inflammatory remodeling and restored homeostatic transcriptional signatures.
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These data identify Reg3g as a modulator of alveolar macrophage functional plasticity during acute lung injury.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE332nnn/GSE332959 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE332959 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1469542 ncbi.nlm.nih.gov/bioproject/PRJNA1469542 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
- Inferred from text
- RNA sequencing 75% · Sequencing 75% · Single-cell RNA sequencing 75%
Provenance · 1 source records, 10 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE332959 | 14 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:single-cell-rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |