Single-nucleus RNA-seq of mouse cortex during mid-gestation pregnancy

This dataset accompanies the study "The lipidomic architecture of the mouse brain" (Fusar Bassini et al.), in which MALDI mass spectrometry imaging was used to map 172 lipids across the adult mouse brain.

The snRNA-seq data were generated to investigate cell type-specific transcriptomic changes in the cortex of pregnant mice, complementing the spatial lipidomics findings. Nuclei were isolated from microdissected cortex using NP-40 lysis and iodixanol gradient purification, and libraries were constructed using the 10x Genomics Chromium Next GEM Single Cell 3' v4 (GEMX) kit. Sequencing was performed on an Element Biosciences AVITI24 system to a depth of ~50,000 reads per nucleus.