Multi-omics expression analysis identifies a panel of secreted markers suitable for in process control cell quality GMP grade productions of keratinocytes from induced pluripotent stem cells

In this study, we present a new and original methodology capable to identify specific temporal biomarkers of keratinocytes differentiation. To do so we combined unbiased single-cell RNA sequencing analysis and quantitative proteomics at multiple stages of keratinocyte differentiation.

This multi-omics analysis identified two secreted markers, IGFBP6 and TNC, capable to precocely monitor the efficacy of epidermal differentiation from the very first’s weeks reducing production costs and later stage failures. Together our results provides valuable insights into the molecular mechanisms involved in epidermal development and highlight the potential to be applied in other cell models for regenerative medicine purposes.