scRNA-seq of bone marrow and splenic emergency granulopoiesis

Here, we isolated bone marrow, spleen and blood Gr1+and cKit+-enriched cells to investigate the dynamics of production across both sites of origin. In addition, we performed the following treatments and blood cells were sorted following similar strategy: (1) Splenectomy: a group of mice was subjected to splenectomy before CPM/AMD treatment; (2) partial irradiation with a shield protecting the abdominal region of the mouse followed by CPM/AMD treatment; (3) Mice treated with CPM/AMD were administered with anti-CXCR2 depleting antibody.