Investigation of epigenetic and transcriptomic features of human B cell development

The study included bulk and single cell epigenetic methodologies coupled with single cell transcriptomics and proteomics. Data included in this submission were generated using the 10x Genomics single-cell RNA, V(D)J Sequencing and Protein feature barcoding method on sorted B cells from adult human blood.

The study demonstrated the emergence of epigenetic differences in B cell subsets at early stages of development that drive divergent developmental paths. Epigenetic features established at early stages are retained in distinct mature cell subpopulations, where they are coupled with transcription of accessible genomic regions. Identified features are retained after cell migration in secondary lymphoid organs.