Omics · study · 2026
Single-cell RNA sequencing of liver non-parenchymal cells from hepatocyte-specific ADK-overexpressing and control mice
Listed in NCBI GEO
Adenosine kinase (ADK) regulates adenosine metabolism and cellular methylation reactions.
Description
This study investigated how hepatocyte-specific ADK overexpression affects the transcriptomic states of liver non-parenchymal cells. Liver non-parenchymal cells were isolated from 5-month-old male hepatocyte-specific ADK-overexpressing mice (Hep-ADKTg) and control mice (Hep-ADKWT) maintained on a standard chow diet.
Single-cell RNA sequencing was performed using the 10x Genomics Chromium platform to characterize liver cell populations and evaluate transcriptional changes in immune and stromal cells. The analysis showed that hepatocyte ADK overexpression promoted proinflammatory transcriptional changes in liver macrophages and monocytes.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE339nnn/GSE339154 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE339154 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1497891 ncbi.nlm.nih.gov/bioproject/PRJNA1497891 ↗
project · from NCBI GEO
- PubMed 36181835 pubmed.ncbi.nlm.nih.gov/36181835 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
- Inferred from text
- Biochemistry and cell biology 69% · RNA sequencing 75% · Sequencing 75% · Single-cell RNA sequencing 75%
Provenance · 1 source records, 11 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE339154 | 14 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].anzsrc:group:3101 | enrichment · NCBI GEO | taxonomy-embedding@1.1.0 | title+keywords+description (69%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:single-cell-rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |