Omics · study · 2026
Single-Cell Transcriptomics Reveals the Characteristics of Bone Marrow Cellular Heterogeneity After Intramedullary Nail Implantation in Osteoporotic Fractures
Listed in NCBI GEO
After fracture, the femoral bone marrow microenvironment in mice initiates a series of reparative responses.
Description
The spatiotemporal coordinated regulation of gene expression not only determines the activation characteristics of hematopoietic and mesenchymal progenitors but also directly regulates the dynamic remodeling of various functional cell populations in the bone marrow microenvironment and the execution of bone repair functions.
Historically, studies on the effects of intramedullary implant materials on the bone marrow microenvironment post-fracture have mostly relied on histological analysis of bulk tissues or functional assays of pre-selected isolated cell subsets. These approaches fail to accurately capture the heterogeneous transcriptional programs of individual cells, especially the dynamic transcriptional changes of cells in the bone marrow and on the implant surface after intramedullary nail placement.
Read the rest (2 more)
To systematically characterize the changes in the femoral bone marrow microenvironment after fracture and clarify the heterogeneity and dynamic transcriptional changes of cells in the bone marrow and on the implant surface following intramedullary nail placement, we employed single-cell mRNA sequencing (scRNA-seq) to profile the transcriptional landscapes of femoral bone marrow samples and implant-adherent cells from fracture mice implanted with intramedullary nails of different materials.
This technique provides core data support for elucidating the molecular mechanisms underlying material-regulated cell differentiation trajectories and functional remodeling during fracture healing.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE313nnn/GSE313141 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE313141 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1378706 ncbi.nlm.nih.gov/bioproject/PRJNA1378706 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
- Inferred from text
- Biochemistry and cell biology 71% · Sequencing 75% · Single-cell RNA sequencing 65%
Provenance · 1 source records, 10 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE313141 | 13 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].anzsrc:group:3101 | enrichment · NCBI GEO | taxonomy-embedding@1.1.0 | title+keywords+description (71%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:single-cell-rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |