Omics · study · 2026
IL7R depletion mitigates neuroinflammation and ischemic stroke via AKT dephosphorylation dependent LCP1 suppression in mice
Listed in NCBI GEO
Stroke remains a leading cause of death and long-term disability worldwide.
Description
After reperfusion following ischemic stroke, peripheral immune cells infiltrate the injured brain hemisphere and exacerbate secondary damage. Among these cells, monocytes are among the predominant population.
In this study, we performed single-cell RNA sequencing of immune cells isolated from the brain 72 h after infarction and characterized the heterogeneity of brain infiltrating monocyte-derived macrophages. We identified an IL7R+ macrophage subset with high fatty-acid metabolism scores 3 days after ischemic stroke in mice. In vitro oxygen glucose deprivation cell model, Il7r expression was significantly increased, accompanied by upregulation of the PI3K-AKT pathway.
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IL7R knockdown decreased phosphorylated Akt and LCP1 levels, and LCP1 knockdown further reduced arachidonic acid, a key mediator of proinflammatory protein translation, as revealed by bulk RNA-seq and metabolomics analyses. Consistent with these in vitro results, IL7R depleted mice exhibited smaller infarct volumes and improved neurological function. Collectively, our findings provide mechanistic insight into how IL7R–pAKT-LCP1 signaling shapes immune-cell metabolism during stroke-induced neuroinflammation and suggest potential therapeutic opportunities targeting immunometabolic pathways in ischemic stroke.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE313nnn/GSE313837 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE313837 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1381653 ncbi.nlm.nih.gov/bioproject/PRJNA1381653 ↗
project · from NCBI GEO
- PubMed 42447806 pubmed.ncbi.nlm.nih.gov/42447806 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
- Inferred from text
- RNA sequencing 75% · Sequencing 75% · Single-cell RNA sequencing 75%
Provenance · 1 source records, 10 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE313837 | 12 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:single-cell-rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |