Omics · study · 2026
Single-cell RNA sequencing of EGFR/RB1/TP53-mutant LUAD cell lines with and without PHOX2B overexpression to assess neuroendocrine phenotype induction
Listed in NCBI GEO
EGFR/RB1/TP53-mutant LUAD cell lines (H1975 and PC9) with and without PHOX2B overexpression were profiled to assess induction of the neuroendocrine (NE) phenotype.
Description
Cell lines transduced with lentiviral PHOX2B cDNA or empty vector (LV105) were analyzed by single-cell RNA sequencing (10x Chromium) with hashtag oligo (HTO) multiplexing (CellPlex). Conditions include parental H1975 and PC9 LUAD cell lines as well as engineered TP53/RB1 double-knockout variants (CYW).
Four biological replicates were performed across 8 pooled sequencing runs (32 demultiplexed samples total).
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE339nnn/GSE339453 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE339453 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1498883 ncbi.nlm.nih.gov/bioproject/PRJNA1498883 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Homo sapiens
- From keywords
- Life Sciences
- Inferred from text
- Biochemistry and cell biology 69% · RNA sequencing 75% · Sequencing 75% · Single-cell RNA sequencing 75%
Provenance · 1 source records, 11 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE339453 | 12 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].anzsrc:group:3101 | enrichment · NCBI GEO | taxonomy-embedding@1.1.0 | title+keywords+description (69%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:single-cell-rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:9606 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |